Place the microplate in the drawer. Multimode Readers Comparison Chart. The SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. 0 500 1000 1500 2000 2500 3000 3500 time (sec. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. NanoRacks added it to their line of commercial . Avoid losing high signal. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. It features a modular design that allows users to add or change detection cartridges according to their needs. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. SoftMax Pro Software’s. Condition: Used - Good. #W50-100) SpectraMax® ABS Plus Microplate Reader. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 54: 1. Growth medium was then replaced with fatty acid uptake solution. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 96-well: 0. Plate Formats: 6 to 384-well microplates. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. Outer wells are aligned for proper viewing with FLIPR. 300 µL of Detergent Compatible Bradford reagent was added to each well. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. To confirm the accurate positioning of arrayed colonies, we performed an absorbance scan in a 96-well scheme with 30 × 30 scans per well for an OmniTray agar plate with 96. Molecular. 96-well: 0. AquaMax 4000 Microplate Washer (Molecular Devices Cat. It also integrates with the. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. In addition, the iD5 reader measures TRF and FP and. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Comparing Luminescence sensitivity of SpectraMax Microplate Readers using ATP Assay | Molecular Devices Author: Molecular DevicesMake the most of your budget with the VersaMax™ ELISA Microplate Reader, our affordable, visible spectrum absorbance reader for 96-well microplates. . Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. 1 OD at 230 nm. Read the microplate Step 1: Place the microplate in the reader. Model:. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Molecular Devices provides. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. VANTAstar™ Flexible Multi-mode Microplate Reader. 0 96 Well Plate. Accuris MR9600 SmartReader 96 Microplate Absorbance Reader. 48 W x 12. 1 mL/well of media. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. i3x. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. adapter plate in drawer of Plate Reader with well A1 in position A1 of the drawer. 1-color 96-well . The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Average StDev (mP) 1. At 5 seconds to read a 96-well endpoint assay, the EMax Microplate Reader has both the speed and performance to carry a heavy workload. Four wash/rinse bottles are included and configurable for both 96- and 384. Specifications for the SpectraMax L microplate reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Reactions catalyzed by firefly and *Renilla luciferases. Multi-Mode Microplate Reader for . A control well was set up to verify. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. explore more. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. microplates or in a cuvette at a. The EMax Plus reader measures flat and round 96-well microplates. This is equivalent to having 511 built-in filters,Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Wavelength Selection. Five-second read per plate. 2 2600A ValitaTiter. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The color touch screen provides a visual user interface, making programming fast and intuitive. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). 100 fmol 1536-well (8 µL) 0. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The microplate reader then generates data following a preconfigured protocol that performs the necessary calculations on the data, delivering consistent results. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Average StDev (mP) 1. /well integration in 96-well luminescence mode and 0. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. the iD5 reader’s filter system. the appropriate well of the microplate. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Figure 2. Reconstitution of reagents. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. #ID3-STD) • L-Tryptophan (Sigma cat. Description. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. for ValitaTiter Plus standards read on Molecular Devices readers. 1 FP • Fluorescence Polarization detection for fluorescein- or . “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. 00. ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. maximum-illumination-channels. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Figure 2. Read More. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Detection by Low noise PMT with simultaneous digital and analog modes. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. The validation kit included individual tubes of diluted fluorophores:. Unparalleled performance on a personalized platform. best-in-class data . fluorescence microplate reader from Molecular Devices. *Simple analysis and optimization. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent) 6. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. #T-0254) Lysozyme (Sigma cat. ValitaTiter Plus 96-well (1 plate) VAL004. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. This reader provides visible. #12-639) White 96- and 384-well microplates (Greiner cat. Microplate Reader (Molecular Devices cat. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. we demonstrate how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. #PLUS 384) Methods. This unlocks the transport slide. StakMax. System setup and calibration are controlled from within SoftMax Pro Software. 04 sec. Determine DNA concentrations precisely and . What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 7. Taking into account the price differences between each seller and each country. The plate reader optics are tunable for. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. 2 mm x 86 mm) Height:. Also refer to this Multimode Reader Comparison Chart. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 2022 Harini Natarajan et al. #3676). 1. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. This reader also makes use of a unique temperature-independent methodology for path length correction. Use standard plate unless you see your type listed. Making it possible to perform kinetic analysis of solid. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 0200-6263. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. Bioz Stars score: 86/100, based on 1 PubMed citations. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. flat meniscus), but is approx 15 mL. 1/17 1907B Printed in USA . +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. Other Molecular Devices readers with absorbance detection mode, e. 8. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. i3x. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. Flexibility to read a variety of plate formats. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. #N207) LAL Reagent Water (Lonza cat. flexibility of monochromator-based systems. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. 2 OD at 200 nm. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesa solid, white, flat-bottom 96-well plate. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 3. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. Wavelength Range 200 to 999 nm. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). Learn more 0200-7008 6- to 1536-well plates. Microplate is validated by molecular devices for the FLIPR reader. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Make sure to Data 96孔微孔板的数据对比. 384-well: 0. Deep well blocks available in 24, 96 and 384-well formatsFlexStation® 3 Multi-Mode Microplate Readers . SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. ValitaTiter Assays Setup Guide 2601A October 2023. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Brand: MOLECULAR DEVICES. Paradigm: Delta mP. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. emax and Vmax microplate readers. Detergent Compatible Bradford assay. #ABS PLUS) Methods. We would like to show you a description here but the site won’t allow us. Well scanning With well scanning, multiple points within each well can be read, providing a high. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Fluorescence Microplate Reader Comparison Chart. g. SpectraMax iD5. Whatever your needs, you’ll find a microplate reader that can meet them. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. ScanLater Western Blot Detection System. SpectraMax 250 User. U-bottom: 30-40 µl. ± 0. Danaher Inc 96 well microplate reader 96 Well Microplate Reader, supplied by Danaher Inc, used in various techniques. 0 fmol/well FITC 200 µL in 96 wells. I have used Molecular Devices’ Vmax 96-well microplate readers since about the time of their inception in the mid to late-1980s. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. Fluorescence Microplate Reader Comparison Chart. Expt#3 . 50 µL of. Bioz Stars score: 86/100, based on 1 PubMed citations. Read time* 96 wells < 15 sec. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. The system is operated through the industry-leading SoftMax® Pro. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 2 mm x 86 mm) Height: between 13. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. #I2511) • Assay diluent – may be cell culture medium. robotics-automation-compatible. 4. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. No additional manuals are available. Description. 96-well microplate at 200 µL/well. validation, maintenance, and repair documentation. • 0. For more sample throughput, the system can well. The CellTiter-Glo assay from Promega uses luciferase. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. Visible absorbance -. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Cross-talk < 0. for ValitaTiter Assays. This . Important things to consider when purchasing a multimode microplate reader. Suggested plates are black walled. We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. 96-well solid bottom, clear polystyrene microplates (Greiner cat. the iD5 reader’s filter system. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Other technologies such as imaging. Fluorescence Microplate Reader Comparison Chart. tools to expand the boundaries of their research. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Description. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. Or scan up to 384 wells at any time to determine the best assay conditions. Transfer of an assay to 96-well microplate technology increases throughput significantly. #I2511)Introduction. g. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Plate Formats: 6 to 384-well microplates. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. Simple, economical . The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Incubate the plate at 37 °C for approximately one hour. 5°C at 37°C. The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). Microplate Reader, Molecular Devices Optimax, Refurbished. (Molecular Devices cat. Materials. Add 150 µL reagent to each well. 7°C and the bottom to 36. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. reagent basin for ease of pipetting into. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The system provides automation for up to 50 microplates for easy walkaway automation. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Microplates . Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Open the catalog to page 5. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Fluorescence Microplate Readers. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Retrieving. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W (ABS Plus) • Option to run samples as low as 2 μL with the SpectraDrop™ Micro-Volume Microplate • Runs various assays within the Vis/UV-Vis range such asI tried Nunc Edge 2. Please click on the links below to view the Microplate Reader Comparison Tables. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. Temperature Control: ambient from +4 to 45C. Ordering information FlexStation 3 Microplate Reader Part Number: FLEX3 • FlexStation 3 Base System Consumables 96-Well, FlexStation Pipet Tips (Black) Part Number: 9000-0911 • 200 µL capacity • Includes 3 vials each of FLIPR Calcium 6 and 6-QF dyes Reagents FLIPR® Calcium 6 Assay Evaluation Kit Part Number. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Microplate readers use a vertical light path so the distance of the. The FilterMax F3 can read 96-well and 384-well plates. 96-well microplate at 200 µL/well. All stand-alone instrument functions can be controlled using the software. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. Microplate luminescent reader assay for ERK activity. 1 sec. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. ©2017 Molecular Devices, LLC . The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Optical System: High energy xenon flash lamp. book Page 9 Wednesday, July 7, 2010 2:09 PM. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. In addition, the iD5 reader measures TRF and FP and. Plate reader instructions Computer. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. iD5. 747. 500 USD. When the user applies a set temperature of 37°C, the top is heated to 37. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Use the provided 3. absorbance, fluorescence, and luminescence with the added functionality of. #iD5-STD) Methods ATP standard curve. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. A. Infinite 200 Pro Microplate Reader. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. #3693; n = 4 for each standard). personalized as your research. g. automation for any lab. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Step 3: Click “Settings” and a screen will appear (Figure 1). /well integration in 384-well luminescence mode Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Plate wash time (300 µl). All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. 2. #SpectraMax L Config) Methods. 4. 4 Pages. 05. As a result, assay flexibility is consequence-free. A preconfigured. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume. Lucid Scientific. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. microplate . Top or bottom detection is available with a simple click of a button. 1700 Online: Sales Offices. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular.